import math import matplotlib.pyplot as plt import numpy as np import squidpy as sq from graph_tool.all import * from src import centrality from src import plot from src import fitting def merfish(): """ Merfish dataset from `squidpy`. """ adata = sq.datasets.merfish() adata = adata[adata.obs.Bregma == -9].copy() return adata def mibitof(): """ Mibitof dataset from `squidpy`. """ adata = sq.datasets.mibitof() return adata def degree(g, weight): # VertexPropertyMap vp = g.new_vertex_property("double") for v in g.vertices(): neighbours = g.get_all_neighbours(v) vp[v] = len(neighbours) return vp def leverage(g, weight): # VertexPropertyMap vp = g.new_vertex_property("double") for v in g.vertices(): li = 0.0 neighbours = g.get_all_neighbours(v) ki = len(neighbours) if ki == 0: continue # sum for nv in neighbours: other_neighbours = g.get_all_neighbours(nv) kj = len(other_neighbours) li += (ki - kj) / (ki + kj) li /= ki vp[v] = li return vp def path(g, weight): # NOTE is this not just betweenness? ep = g.new_vertex_property("double") for v in g.vertices(): for u in g.vertices(): if (v == u): continue paths = graph_tool.topology.all_shortest_paths(g, v, u, weights=weight, edges=True) for edges in paths: for edge in edges: for idx, g_e in enumerate(g.edges()): if (g_e == edge): # NOTE we end up counting twice! ep[idx] += 0.5; break # for e in g.edges(): # ep[e] /= 2; return ep def random_graph(n=5000, seed=None): """ Uniformly random point cloud generation. `n` [int] Number of points to generate. Default 5000 seems like a good starting point in point density and corresponding runtime for the subsequent calculations. @return [numpy.ndarray] Array of shape(n, 2) containing the coordinates for each point of the generated point cloud. """ if seed is None: import secrets seed = secrets.randbits(128) rng = np.random.default_rng(seed=seed) return rng.random((n, 2)), seed def spatial_graph(adata): """ Generate the spatial graph using delaunay for the given `adata`. `adata` will contain the calculated spatial graph contents in the keys adata.obsm['spatial']` in case the `adata` is created from a dataset of *squidpy*. @return [Graph] generated networkx graph from adata.obsp['spatial_distances'] """ g, pos = graph_tool.generation.triangulation(adata, type="delaunay") g.vp["pos"] = pos weight = g.new_edge_property("double") for e in g.edges(): weight[e] = math.sqrt(sum(map(abs, pos[e.source()].a - pos[e.target()].a)))**2 return g, weight def apply(g, seed, weight, convex_hull, ax, method, method_name): # calculate centrality values ep = None if method_name == "Betweeness": vp, ep = method(g, weight=weight) else: ep = method(g, weight=weight) ep.a = np.nan_to_num(ep.a) # correct floating point values # normalization min_val, max_val = ep.a.min(), ep.a.max() ep.a = (ep.a - min_val) / (max_val - min_val) quantification = plot.quantification_data_edges(g, ep, convex_hull) # optimize model's piece-wise linear function d = quantification[:, 0] C = quantification[:, 1] m_opt, c0_opt, b_opt, aic_opt = fitting.fit_piece_wise_linear(d, C) # TODO # should this be part of the plotting function itself, it should not be necessary for me to do this d_curve = np.linspace(min(d), max(d), 500) C_curve = np.piecewise( d_curve, [d_curve <= b_opt, d_curve > b_opt], [lambda x: m_opt * x + c0_opt, lambda x: m_opt * b_opt + c0_opt] ) # plot model containing modeled piece-wise linear function plot.quantification_plot(ax, quantification, d_curve, C_curve, method_name, aic_opt) # # - Create a random point cloud and calculate a triangulation on it # - For that graph calculate the convex hull # - Draw the graph with the convex hull # - For each centrality measure # - apply centrality measure to the next axis # - Draw the corresponding resulting models into a grid # points, seed = random_graph(n=5000) g, weight = spatial_graph(points) g = GraphView(g) # calculate convex hull convex_hull = centrality.convex_hull(g) # plot graph with convex_hull fig_graph, ax_graph = plt.subplots(figsize=(15, 12)) # draw without any centrality measure `ep` ep = g.new_edge_property("double") plot.graph_plot(fig_graph, ax_graph, g, ep, convex_hull, f"Pointcould (seed: {seed})", True) # draw edges fig_graph.savefig(f"comparison_edge_scores_artificial_graph.svg", format='svg') fig = plt.figure(figsize=(15, 12)) row1, row2 = fig.subplots(2, 4) # TODO run, betweenness, k-path, eigenedge, etc. # - some share similarities to the node based counter parts ax1, ax2, ax3, ax4 = row1 apply(g, None, weight, convex_hull, ax1, betweenness, "Betweeness") # ax1, ax2, ax3, ax4 = row2 # apply(g, None, weight, convex_hull, ax1, katz, "Katz") # apply(g, None, weight, convex_hull, ax2, hits, "Hits") # apply(g, None, weight, convex_hull, ax3, leverage, "Leverage") # apply(g, None, weight, convex_hull, ax4, degree, "Degree") fig.savefig(f"Comparison_edge_centralities_artificial_.svg", format='svg')